How the Images Were Acquired and Processed

Images in the Loan Slide and D-Series Slide Folders were captured from microscope slides in the "Master" sets of each collection using a FUJIFILM FUJIX HC-300Z Digital Camera. Images were captured directly to ZipDisk using the on-board software of the FUJIX camera, then transferred to my Desktop computer (MacIntosh G-3) for processing using Tiffany software. For the lowest magnification images, the FUJIX was mounted on an adjustable stand that was positioned above the stage of a Zeiss Photomicroscope from which all optical components except the light source had been removed. Light from the substage illuminator was passed through a milk glass diffusor and then served to transilluminate each slide. A macro lens (Computar, 5.5mm telecentric) allowed the capture of a wide-enough field (19 mm) that almost all the tissues on most slides was imaged. For many slides the zoom features of the macro lens were used to capture smaller fields (as low as 7 mm) so that the smallest slide materials could be displayed almost filling the image frame.
For all higher magnification images, the FUJIX was coupled (C-mount) to a Zeiss Photomicroscope equipped with standard bright field optics. With an optivar setting of 1.25X, full frame widths were 2400, 1000, 395, 165, and 100 um with objectives of 2.5x, 6.3x, 16x, 40x, and 63x, respectively.
Using Tiffany software, the image files were converted to JPEG at 72 dpi and resized to give both full-screen and thumbnail versions, which were then ported to the web server. Although the captured image files (tiff) and the JPEG files derived from them were of very high quality, both as to color accuracy and "resolution", in some cases it was desirable to use the Tiffany software to adjust contrast and/or brightness slightly, and/or to use a crisping function to sharpen the images somewhat. When such image processing was done, it was performed so that the image on the computer monitor looked as much as possible like the microscope slide as viewed either by the naked eye or in the oculars of the Photomicroscope.

Since multiple images are captured for each slide "folder" (different versions of many slides, various magnifications), it was necessary to devise and use a routine file labeling system. The one in use is as follows:
1. All images related to a single slide folder (e.g. H37, D12) are placed in the same "Images" folder.
2. When multiple versions of the same slide exist, they are numbered as, e.g. H37a, H37b, etc.
3. Very low magnification views (macro lense) are labelled from 01 to 09, e.g. H37a01 or H37b03.
4. Images captured with the 2.5X lense of the photomicroscope are labeled from 10 to 19, e.g. H37a10 or H37b15.
5. The numbering for higher magnification lenses proceeds upwards in decades; hence images taken with the 6.3X, 16X, 40X or 63X are numbered 20-29, 30-39, 40-49, and 50-59, respectively.

Where relocation of specific fields on slides was deemed necessary, use was made of commercially available Finder Slides.

I am especially grateful to Dr. Jozsef Czege for much assistance with the FUJIX camera and my G-3 in general and with the use of Tiffany software in particular.

Back to the Loan Slide Collection Overview Page

Last modified: Wednesday, February 4, 2009.